A combination of two Brugia malayi filarial vaccine candidate antigens (BmALT-2 and BmVAH) enhances immune responses and protection in jirds.
Identifieur interne : 005134 ( Main/Exploration ); précédent : 005133; suivant : 005135A combination of two Brugia malayi filarial vaccine candidate antigens (BmALT-2 and BmVAH) enhances immune responses and protection in jirds.
Auteurs : S B Anand [Inde] ; K N Kodumudi ; M V Reddy ; P. KalirajSource :
- Journal of helminthology [ 1475-2697 ] ; 2011.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antihelminthe (sang), Antigènes d'helminthe (génétique), Antigènes d'helminthe (immunologie), Antigènes d'helminthe (métabolisme), Brugia malayi (génétique), Brugia malayi (immunologie), Brugia malayi (pathogénicité), Cellules CHO, Cricetinae, Filariose lymphatique (), Filariose lymphatique (immunologie), Gerbillinae (immunologie), Immunisation, Immunoglobuline G (sang), Modèles animaux de maladie humaine, Mâle, Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Protéines recombinantes (métabolisme), Vaccins à ADN (administration et posologie), Vaccins à ADN (génétique), Vaccins à ADN (immunologie).
- MESH :
- administration et posologie : Vaccins à ADN.
- génétique : Antigènes d'helminthe, Brugia malayi, Protéines recombinantes, Vaccins à ADN.
- immunologie : Antigènes d'helminthe, Brugia malayi, Filariose lymphatique, Gerbillinae, Protéines recombinantes, Vaccins à ADN.
- métabolisme : Antigènes d'helminthe, Protéines recombinantes.
- pathogénicité : Brugia malayi.
- sang : Anticorps antihelminthe, Immunoglobuline G.
- Animaux, Cellules CHO, Cricetinae, Filariose lymphatique, Immunisation, Modèles animaux de maladie humaine, Mâle.
English descriptors
- KwdEn :
- Animals, Antibodies, Helminth (blood), Antigens, Helminth (genetics), Antigens, Helminth (immunology), Antigens, Helminth (metabolism), Brugia malayi (genetics), Brugia malayi (immunology), Brugia malayi (pathogenicity), CHO Cells, Cricetinae, Disease Models, Animal, Elephantiasis, Filarial (immunology), Elephantiasis, Filarial (prevention & control), Gerbillinae (immunology), Immunization, Immunoglobulin G (blood), Male, Recombinant Proteins (genetics), Recombinant Proteins (immunology), Recombinant Proteins (metabolism), Vaccines, DNA (administration & dosage), Vaccines, DNA (genetics), Vaccines, DNA (immunology).
- MESH :
- chemical , administration & dosage : Vaccines, DNA.
- chemical , blood : Antibodies, Helminth, Immunoglobulin G.
- chemical , genetics : Antigens, Helminth, Recombinant Proteins, Vaccines, DNA.
- chemical , immunology : Antigens, Helminth, Recombinant Proteins, Vaccines, DNA.
- chemical , metabolism : Antigens, Helminth, Recombinant Proteins.
- genetics : Brugia malayi.
- immunology : Brugia malayi, Elephantiasis, Filarial, Gerbillinae.
- pathogenicity : Brugia malayi.
- prevention & control : Elephantiasis, Filarial.
- Animals, CHO Cells, Cricetinae, Disease Models, Animal, Immunization, Male.
Abstract
In this study filarial recombinant protein or DNA vaccine constructs encoding BmALT-2 and BmVAH as single or as cocktail antigens were evaluated. Male jirds were immunized intramuscularly with DNA vaccine constructs or were immunized intraperitoneally with protein vaccine. The single and bicistronic DNA constructs induced substantial interferon-γ responses in spleen cells; antigen-specific responses were higher following immunization with the bicistronic cocktail construct and evoked a significant protective response of 57% in jirds challenged with Brugia malayi that was similar in the antibody-dependent cellular cytotoxicity (ADCC) assay and micropore chamber experiment. The cocktail protein vaccines induced a mixture of IgG2a (Th1) and IgG1 (Th2) responses with 80% protective response when challenged with B. malayi infective larvae. However, the single protein vaccine rALT-2 induced Th2 (IgG1/IgG3) with a 70% protective response and rVAH induced Th1 (IgG2a) with a lower proliferative response with 60% protection following challenge with B. malayi infective larvae. These results suggest that filarial cocktail protein vaccines are able to elicit substantial immune and protective responses when compared with single antigen vaccination in suitably vaccinated jirds.
DOI: 10.1017/S0022149X10000799
PubMed: 21208482
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002787
- to stream PubMed, to step Curation: 002787
- to stream PubMed, to step Checkpoint: 002787
- to stream Ncbi, to step Merge: 004056
- to stream Ncbi, to step Curation: 004056
- to stream Ncbi, to step Checkpoint: 004056
- to stream Main, to step Merge: 005173
- to stream Main, to step Curation: 005134
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A combination of two Brugia malayi filarial vaccine candidate antigens (BmALT-2 and BmVAH) enhances immune responses and protection in jirds.</title><author><name sortKey="Anand, S B" sort="Anand, S B" uniqKey="Anand S" first="S B" last="Anand">S B Anand</name><affiliation wicri:level="1"><nlm:affiliation>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai - 625021, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai - 625021</wicri:regionArea><wicri:noRegion>Madurai - 625021</wicri:noRegion></affiliation></author><author><name sortKey="Kodumudi, K N" sort="Kodumudi, K N" uniqKey="Kodumudi K" first="K N" last="Kodumudi">K N Kodumudi</name></author><author><name sortKey="Reddy, M V" sort="Reddy, M V" uniqKey="Reddy M" first="M V" last="Reddy">M V Reddy</name></author><author><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2011">2011</date><idno type="RBID">pubmed:21208482</idno><idno type="pmid">21208482</idno><idno type="doi">10.1017/S0022149X10000799</idno><idno type="wicri:Area/PubMed/Corpus">002787</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002787</idno><idno type="wicri:Area/PubMed/Curation">002787</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002787</idno><idno type="wicri:Area/PubMed/Checkpoint">002787</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002787</idno><idno type="wicri:Area/Ncbi/Merge">004056</idno><idno type="wicri:Area/Ncbi/Curation">004056</idno><idno type="wicri:Area/Ncbi/Checkpoint">004056</idno><idno type="wicri:Area/Main/Merge">005173</idno><idno type="wicri:Area/Main/Curation">005134</idno><idno type="wicri:Area/Main/Exploration">005134</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">A combination of two Brugia malayi filarial vaccine candidate antigens (BmALT-2 and BmVAH) enhances immune responses and protection in jirds.</title><author><name sortKey="Anand, S B" sort="Anand, S B" uniqKey="Anand S" first="S B" last="Anand">S B Anand</name><affiliation wicri:level="1"><nlm:affiliation>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai - 625021, India.</nlm:affiliation><country xml:lang="fr">Inde</country><wicri:regionArea>Department of Genetic Engineering, School of Biotechnology, Madurai Kamaraj University, Madurai - 625021</wicri:regionArea><wicri:noRegion>Madurai - 625021</wicri:noRegion></affiliation></author><author><name sortKey="Kodumudi, K N" sort="Kodumudi, K N" uniqKey="Kodumudi K" first="K N" last="Kodumudi">K N Kodumudi</name></author><author><name sortKey="Reddy, M V" sort="Reddy, M V" uniqKey="Reddy M" first="M V" last="Reddy">M V Reddy</name></author><author><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name></author></analytic><series><title level="j">Journal of helminthology</title><idno type="eISSN">1475-2697</idno><imprint><date when="2011" type="published">2011</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Helminth (blood)</term><term>Antigens, Helminth (genetics)</term><term>Antigens, Helminth (immunology)</term><term>Antigens, Helminth (metabolism)</term><term>Brugia malayi (genetics)</term><term>Brugia malayi (immunology)</term><term>Brugia malayi (pathogenicity)</term><term>CHO Cells</term><term>Cricetinae</term><term>Disease Models, Animal</term><term>Elephantiasis, Filarial (immunology)</term><term>Elephantiasis, Filarial (prevention & control)</term><term>Gerbillinae (immunology)</term><term>Immunization</term><term>Immunoglobulin G (blood)</term><term>Male</term><term>Recombinant Proteins (genetics)</term><term>Recombinant Proteins (immunology)</term><term>Recombinant Proteins (metabolism)</term><term>Vaccines, DNA (administration & dosage)</term><term>Vaccines, DNA (genetics)</term><term>Vaccines, DNA (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antihelminthe (sang)</term><term>Antigènes d'helminthe (génétique)</term><term>Antigènes d'helminthe (immunologie)</term><term>Antigènes d'helminthe (métabolisme)</term><term>Brugia malayi (génétique)</term><term>Brugia malayi (immunologie)</term><term>Brugia malayi (pathogénicité)</term><term>Cellules CHO</term><term>Cricetinae</term><term>Filariose lymphatique ()</term><term>Filariose lymphatique (immunologie)</term><term>Gerbillinae (immunologie)</term><term>Immunisation</term><term>Immunoglobuline G (sang)</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term><term>Protéines recombinantes (génétique)</term><term>Protéines recombinantes (immunologie)</term><term>Protéines recombinantes (métabolisme)</term><term>Vaccins à ADN (administration et posologie)</term><term>Vaccins à ADN (génétique)</term><term>Vaccins à ADN (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="administration & dosage" xml:lang="en"><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Helminth</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Helminth</term><term>Recombinant Proteins</term><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Helminth</term><term>Recombinant Proteins</term><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Helminth</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="administration et posologie" xml:lang="fr"><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Protéines recombinantes</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Brugia malayi</term><term>Filariose lymphatique</term><term>Gerbillinae</term><term>Protéines recombinantes</term><term>Vaccins à ADN</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Brugia malayi</term><term>Elephantiasis, Filarial</term><term>Gerbillinae</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes d'helminthe</term><term>Protéines recombinantes</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Brugia malayi</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Elephantiasis, Filarial</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antihelminthe</term><term>Immunoglobuline G</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>CHO Cells</term><term>Cricetinae</term><term>Disease Models, Animal</term><term>Immunization</term><term>Male</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules CHO</term><term>Cricetinae</term><term>Filariose lymphatique</term><term>Immunisation</term><term>Modèles animaux de maladie humaine</term><term>Mâle</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In this study filarial recombinant protein or DNA vaccine constructs encoding BmALT-2 and BmVAH as single or as cocktail antigens were evaluated. Male jirds were immunized intramuscularly with DNA vaccine constructs or were immunized intraperitoneally with protein vaccine. The single and bicistronic DNA constructs induced substantial interferon-γ responses in spleen cells; antigen-specific responses were higher following immunization with the bicistronic cocktail construct and evoked a significant protective response of 57% in jirds challenged with Brugia malayi that was similar in the antibody-dependent cellular cytotoxicity (ADCC) assay and micropore chamber experiment. The cocktail protein vaccines induced a mixture of IgG2a (Th1) and IgG1 (Th2) responses with 80% protective response when challenged with B. malayi infective larvae. However, the single protein vaccine rALT-2 induced Th2 (IgG1/IgG3) with a 70% protective response and rVAH induced Th1 (IgG2a) with a lower proliferative response with 60% protection following challenge with B. malayi infective larvae. These results suggest that filarial cocktail protein vaccines are able to elicit substantial immune and protective responses when compared with single antigen vaccination in suitably vaccinated jirds.</div></front></TEI><affiliations><list><country><li>Inde</li></country></list><tree><noCountry><name sortKey="Kaliraj, P" sort="Kaliraj, P" uniqKey="Kaliraj P" first="P" last="Kaliraj">P. Kaliraj</name><name sortKey="Kodumudi, K N" sort="Kodumudi, K N" uniqKey="Kodumudi K" first="K N" last="Kodumudi">K N Kodumudi</name><name sortKey="Reddy, M V" sort="Reddy, M V" uniqKey="Reddy M" first="M V" last="Reddy">M V Reddy</name></noCountry><country name="Inde"><noRegion><name sortKey="Anand, S B" sort="Anand, S B" uniqKey="Anand S" first="S B" last="Anand">S B Anand</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Sante/explor/LymphedemaV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005134 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005134 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Wicri/Sante
|area= LymphedemaV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:21208482
|texte= A combination of two Brugia malayi filarial vaccine candidate antigens (BmALT-2 and BmVAH) enhances immune responses and protection in jirds.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:21208482" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a LymphedemaV1
| This area was generated with Dilib version V0.6.31. |  |